Note
L00436 interferes with IgG in mouse/rat serum and is not suitable for detecting mouse/rat serum samples.
| Product Description |
GenScript His
Tag ELISA Detection Kit, a 1.5 hour competition
ELISA, is developed for rapid and high
throughput detection of His-tagged proteins. There are several potential usages:
●Quickly identify the
presence of His-tagged proteins in samples.
●Optimize protein
expression by monitoring the His-tagged proteins level.
●High throughput screening of
stable cell lines expressing His-tagged proteins.
|
| Sensitivity |
1 ng/ml His-tagged proteins |
| Detection Range |
1 ng/ml~729 ng/ml |
| Test Samples |
N-terminal/C-terminal/internal His-tagged proteins4 x His/5 x His/6 x His-tagged proteins
Mammalian, yeast and bacteria cell lysates or cell supernatant |
| Conveniency |
Provide all reagents required for test
Complete test within
1.5 hours |
| Kit Contents |
| Components |
Quantity |
Part.No |
| His Tag Plate |
1 plate (8 wells x 12 strips) |
436-80 |
| Anti-His Monoclonal Antibody |
6 ml |
436-20 |
| Antibody Tracer |
12 ml |
436-30 |
| His Tag Standards
(0,1,3,9,27,81,243,729 ng/ml) |
1 ml per each standard |
436-11,12,13,14,15,16,17,18 |
| Assay Diluent |
60 ml |
436-60 |
| 20 × Wash Solution |
40 ml |
B-A1-70 |
| TMB Solution |
12 ml |
B-A1-40 |
| Stop Solution |
6 ml |
B-A1-50 |
| His Tag Standard Stock (10 μg/ml) |
500 μl |
436-10 |
| Plate Sealer |
2 |
N/A |
| User Manual |
1 |
N/A |
|
| Reagents compatibility |
Tolerable
with various reagents at certain concentration |
| Storage |
The unopened kit is stable for
at least 12 months if stored at 2-8 °C
and the opened kit may be stable for up to 1 month at 2-8 °C. Do not freeze the kit. |
Concentration gradient represents X, OD450 value represents Y, all X values will be transformed to Log(X) to create the standard curve which was calculated by 4PL (Figure 1). The operator can get log values of sample concentration by inputting the OD450 value of unknown samples. By formula X=10X, sample concentration can be calculated.
The intra-assay precision of the L00436 is less than 10%, and the inter-assay precision is less than 15%.
For research use only. Not intended for
human or animal clinical trials, therapeutic or diagnostic use.
